Improvement of NRF2 gene expression and antioxidant status in patients with type 2 diabetes mellitus after supplementation with omega-3 polyunsaturated fatty acids: A double-blind randomised placebo-controlled clinical trial

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Pegah Golpour 1, Mitra Nourbakhsh 2, Maryam Mazaherioun 3, Leila Janani 4, Mona Nourbakhsh 5, Parichehreh Yaghmaei 1

Main idea: Supplementation with n-3 PUFAs enhanced NRF2 gene expression and improved overall antioxidant capacity and thus might be considered beneficial in the amelioration of oxidative stress and prevention of T2DM complications.

Abstract

Background: Nrf2 is a transcription factor that induces the expression of several proteins with antioxidant properties such as sestrin2 (Sesn2) and is therefore considered as the major regulator of anti-oxidative defence system.

Objectives: The aim of this research was to study the effect of supplementation with n-3 PUFAs on the antioxidant status and the gene expression of Nrf2 and Sestrin2 (Sesn2) in patients with type 2 diabetes mellitus (T2DM).

Participants: Sixty patients with T2DM were enrolled in a placebo-controlled, double-blind, randomised clinical trial.

Intervention and design: Study tested the expression of Sesn2 and Nrf2 genes in peripheral blood mononuclear cells. Total antioxidant status in plasma samples was also measured based on the ferric reducing ability of plasma. The participants were randomly allocated to two intervention groups receiving n-3 PUFAs or placebo soft gels.

Results: NRF2 gene expression was significantly increased in n-3 PUFA-supplemented subjects, compared with the placebo group. Plasma total antioxidant status was also significantly augmented. SESN2 gene expression was not significantly affected.

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