Different therapeutic effects between diabetic and non-diabetic adipose stem cells in diabetic wound healing

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Abstract

This study aimed to investigate how adipose tissue-derived stem cells (ASCs) from diabetic and from non-diabetic rats affect wound healing in different microenvironments. Adipose-derived stem cells (ASCs) are mesenchymal stem cells (MSCs) that are obtained from abundant adipose tissue, adherent on plastic culture flasks, can be expanded in vitro, and have the capacity to differentiate into multiple cell lineages.

The two types of Adipose-derived stem cells-rich cells were distinguished by characteristic surface antigen detection. The Adipose-derived stem cells-rich cells were transplanted into the wounds of diabetic and non-diabetic rats. Wound healing rates were compared and the healing process in the wound margin sections was used to determine how Adipose-derived stem cells-rich cells affect wound healing in different microenvironments.

Adipose-derived stem cells density was decreased in diabetic rats. The generation time of Adipose-derived stem cells-rich cells from diabetic rats (d-ASC-rich cells) was longer than that of Adipose-derived stem cells-rich cells from non-diabetic rats. In non-diabetic mice these cells had lower mortality rate and inflammation markers. Transplantation of ASC-rich cells from non-diabetic rats promoted wound healing in both non-diabetic and diabetic rats. In contrast, Adipose-derived stem cells and enriched nuclear cells only promoted wound healing in non-diabetic rats. Adipose-derived stem cells-rich cell transplantation promoted greater tissue regeneration than Adipose-derived stem cells-rich cell transplantation.

Conclusion: Adipose-derived stem cells-rich cells promoted wound healing in diabetic and non-diabetic rats. Adipose-derived stem cells density was lower in the adipose tissue of diabetic rats compared with non-diabetic rats. Adipose-derived stem cells-rich cells did not promote wound healing in diabetic rats, suggesting that caution is warranted regarding the clinical use of diabetic adipose stem cell transplantation for the treatment of diabetic wounds.

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